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KMID : 1094720000050060436
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Biotechnology and Bioprocess Engineering
2000 Volume.5 No. 6 p.436 ~ p.440
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Biodetoxification of coumaphos insecticide using immobilized Escherichia coli expressing organophosphorus hydrolase enzyme on cell surface
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Mansee Ayman H.
Chen Wilfred
Mulchandani Ashok
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Abstract
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Recently, we reported an improved technology for the degradation of organophosphate nerve agents using whole cells of genetically engineeredEscherichia coli that anchored and displayed the enzyme organophosphorus hydrolase on the cell surface. In this paper we report the immobilization of these cells on highly porous sintered glass beads and the subsequent application of the immobilized cell in a continuous-flow packed bed bioreactor for the biodetoxification of a widely used insecticide, coumaphos.
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KEYWORD
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OPH, pesticides, nerve agents, bioreactor, porous sintered glass beads
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